Frozen Tissues

“Frozen sections are one of the most important and difficult procedures a pathologist performs during his practice. It requires experience, knowledge of clinical pathology, the capacity to make quick decisions under pressure and a keen awareness of the limitations» says Prof. Juan Rosai, one of the most eminent pathologists in the world, author of the leading treatise on the specialty entitled, “Rosai and Ackerman’s Surgical Pathology”.

On the surgery table, now more than ever, patients are in need of quick/reliable answers about how the surgical operation is going.
The so-called Frozen Section Procedure is an urgent procedure, known to offer slides of poor morphological quality due to the slow freezing procedure.

frozen tissues

PrestoCHILL reduces freezing time to 60 seconds and eliminates freezing artifacts through ultrafast freezing, preventing the formation of ice crystals. Cut fatty tissues, like breast or lymph nodes, can be frozen without difficulty and without the distortion of their delicate honeycomb structures.

While using Milestone’s patented “face-down” embedding technique and PrestoCHILL, it is possible to obtain perfectly “flat plane” surfaces to reduce trimming time and cut more easily.

frozen tissues

The high quality of Milestone frozen sections can only be obtained by freezing the specimens with PrestoCHILL and by processing the slides with FineFIX before staining. The FineFIX Module consists of a heated metal vessel in which the FineFIX solution is kept at 40°C.

frozen tissues

PRESTO PRO is an innovative benchtop processor/stainer for high quality frozen sections. It introduces a standardized approach to processing slides using FineFIX, a patented Milestone solution, followed by fully automated staining protocols.

It is now possible to produce frozen sections with permanent-like quality by combining the artifact-free tissue freezing of PrestoCHILL with the outstanding features of the all-new PRESTO PRO processing/staining unit.

frozen tissues

Over the past decade or so, it has become clear that the great majority of biospecimens stored in the world’s biorepositories may not be suitable for the state-of-the-art genomics, proteomics, metabolomics and other bioanalytical technologies used today to search for cancer-related biomarkers.
The irreproducibility of many reported biomarkers is due, at least in part, to the fact that the biospecimens utilized are often procured using different collection, processing and storage techniques.

To overcome some of these difficulties, Milestone has developed FlashFREEZE, which standardizes the flash-freezing processing step for biospecimens. With FlashFREEZE, it is now possible to carry out evidence-based freezing protocols on biospecimens with the full documentation needed for QA purposes.